Description
Feature
1. 15 Minutes Protocol
2. 3 Simple Steps: Chip Setup, Sample Injection, Detection
3. Label-Free Patented Technology
4. Portable Device with Affordable Chips & Assay Kits
5. Picogram Level Sensitivity
How Does It Work?
The sensing mechanism of the highly sensitive fiber optic-particle plasmon resonance sensor (FOPPR) relies on interrogating the plasmonic scattering of light from gold nanoparticles (AuNPs). The scattered lights on the optic fiber is a response due to a change in the refractive index or molecular binding event in real-time.
Experimenral Results:
Sucrose
Temporal response of the biosensor signal with serial injection of samples with refractive index of (A) ddH₂O, (B) 6.8% sucrose, (C) 13.25% sucrose, (D) 19.45% sucrose, (E) 25.4% sucrose, (F) 31.05% sucrose, (G) 36.25% sucrose, (H) 41.7% sucrose and (I-K) ddH₂O. The measuring time interval was 300 sec.
Immunoglobin G (IgG)
Binding of (A) 8 ×10-7 g/mL, (B) 1 ×10-6 g/mL, (C) 2 ×10-6 g/mL, (D) 4 ×10-6 g/mL, (E) 6 ×10-6 g/mL, (F) 8 ×10-6 g/mL, (G) 1 ×10-5 g/mL of 150 kDa anti-IgG (goat polyclonal antibody) and (H) PBS to 150 kDa IgG (mouse polyclonal antibody) that was conjugated onto AuNPs surface. The measuring time interval was 900 sec.
The sensing strategy of the highly sensitive fiber optic-particle plasmon resonance sensor (FO-PPR) relies on interrogating the plasmonic scattering of light from gold nanoparticles (AuNPs) on the optical fiber in response to the surrounding refractive index changes or molecular binding events in real-time.
Filux C1 Application Note:
CeNP Surface Properties and their Binding Kinetics to Immunoglobins in Mouse Plasma
