Chemlux

Description

The Chemlux Imager is a powerful, easy-to-use, full-featured standalone imaging system for fluorescent/luminescent/colorimetric signal acquisition from gels or blots via our advanced lighting/imaging technology and proprietary integrated software.  Equipped with a 6 M cooled CCD camera (-550C ± 50 ambient) along with an absolute sealed darkroom, the system is able to capture the chemiluminescence signal from Western blot ECL images for up to 90 min. The system also houses various light source settings to allow imaging of proteomic 2D DIGE and to capture fluorescent signals excited by different light sources such as UV, red, green, blue and white light.  Chemlux’s unique UI design allows the user to intuitively obtain the image data without any difficulty. The Chemlux Imager is an advanced gel documentation system designed to fully meet all your gel/blot imaging needs.
 

Feature

1.High Resolution and High Sensitivity of 6 M Cooled (-550C ± 50 ambient) CCD Camera
2.16-bit, High Dynamic Range, up to 6 X 6 Bin Mode
3.Four-level Movable Platform with Auto Focus Feature
4.Adjustable RGB light intensity to get optimized image
5.Cooling status indication on UI
6.Auto back up to prevent important data loss
7.Remote operation via LAN/WLAN login

Chemiluminescence Application

Western Blot
Chemiluminescence ECL Detection
Exposure time: 30 min

No noise was observed after 30 min exposure time.

Arrow indicates a 120 ng of protein sample.

THP1 cell line sample starting from 15 ug to 120 ng (5X in serial dilution) were prepared and loaded for SDS-PAGE. Proteins were transferred onto nitrocellulose membrane for further Western Blot analysis. ECL substrate kit was applied on the membrane.
    Western Blot
Chemiluminescence ECL+ (ng-fg level) Detection
Exposure time: 3 min

Arrow indicated a 58.6 ng of protein sample.

THP1 cell line sample starting from 7.5 ug to 58.6 ng (2X in serial dilution) were prepared and loaded for SDS-PAGE. Proteins were transferred onto nitrocellulose membrane for further Western Blot analysis. ECL+ (ng-fg level) kit was applied on the membrane.

 

Colorimetric Application

Western Blot
HRP/DAB Treatment
Exposure time: 30 ms
Epi White Light Setting
SDS-PAGE
Coomassie Blue Treatment
Exposure time: 30 ms
White Backlight Setting

Fluorescence Applications (DNA-based)

DNA Agarose Gel
EtBr Stain
UV light Excitation
Lower Level
200 ms
DNA Agarose Gel
ECO Safe Stain
Epi Blue Light Excitation
Mid Level
200 ms

Fluorescence Applications (Protein-based)

SDS-PAGE
Alexa 488 Stain
Epi Blue Light Excitation
Mid Level
5000 ms
SDS-PAGE
Alexa 546 Stain
Epi Green Light Excitation
Mid Level
2000 ms
SDS-PAGE
Alexa 647 Stain
Epi Red Light Excitation
Mid Level
200 ms

Specifications

  Chemlux 600 W Chemlux 600 BW% Chemlux 600 RGBW*
Control Panel Display 10.4" Multi-touch LCD Panel 10.4" Multi-touch LCD Panel 10.4" Multi-touch LCD Panel
Touch Panel Cap Sense Touch Panel Cap Sense Touch Panel Cap Sense Touch Panel
OS Embedded Linux Embedded Linux Embedded Linux
Camera Sensor 6M CCD 6M CCD 6M CCD
Resolution 2,736 X 2,160 Pixels 2,736 X 2,160 Pixels 2,736 X 2,160 Pixels
Pixel Size 4.54 mm x 4.54 mm 4.54 mm x 4.54 mm 4.54 mm x 4.54 mm
Exposure time 10 ms – 90 min 10 ms – 90 min 10 ms – 90 min
Cooling Temperature  -550C ± 50C from Ambient -550C ± 50C from Ambient -550C ± 50C from Ambient
Optical Lens 1"  25 mm Fixed Focal Length 1"  25 mm Fixed Focal Length 1"  25 mm Fixed Focal Length
Auto Focus Yes (Auto) Yes (Auto) Yes (Auto)
IRIS F0.95 F0.95 F0.95
Zoom 3- stage position 3- stage position 3- stage position
1. 12 cm x 9 cm 1. 12 cm x 9 cm 1. 12 cm x 9 cm
2. 19 cm x 14 cm 2. 19 cm x 14 cm 2. 19 cm x 14 cm
3. 26 cm x 20 cm 3. 26 cm x 20 cm 3. 26 cm x 20 cm
Manual operation Manual operation Manual operation
Filter 6 Position (Motor-powered) 6 Position (Motor-powered) 6 Position (Motor-powered)
Viewing Area 26 cm x 20 cm (Maximum) 26 cm x 20 cm (Maximum) 26 cm x 20 cm (Maximum)
Light Source UV@ 306 nm UV Trans-illuminator (For Chemlux 600 W-UV only)   306 nm UV Trans-illuminator (For Chemlux 600 BW-UV only)  306 nm UV Trans-illuminator (For Chemlux 600 RGBW-UV only)  
White Backlight Plate Yes, 26 cm x 20 cm Yes, 26 cm x 20 cm Yes, 26 cm x 20 cm
EPI RGB   N/A EPI Blue light  EPI R/G/B light
EPI White Light Yes Yes Yes
Lift Plate 3 Lift Level Manual Adjustable Manual Adjustable Manual Adjustable
Connection USB 2.0 Port X3 (Front x1; Rear x2) X3 (Front x1; Rear x2) X3 (Front x1; Rear x2)
10/100 Ethernet Port X1 X1 X1
Capture Capture Mode UV (UV Series only) UV (UV Series only) UV (UV Series only)
White backlight White backlight White backlight
N/A EPI blue EPI R/G/B light
Chemiluminescent (Dark Room) Chemiluminescent (Dark Room) Chemiluminescent (Dark Room)
EPI white EPI white EPI white
Image Format TIFF 16 Bit/ JPG/BMP TIFF 16 Bit/ JPG/BMP TIFF 16 Bit/ JPG/BMP
Storage 8 GB  Data storage 8 GB  Data storage 8 GB  Data storage
Certificates CE & FCC CE & FCC Certified CE & FCC Certified CE & FCC Certified
Dimensions L x W x H 452 X 423 X 659 mm 452 X 423 X 659 mm 452 X 423 X 659 mm
Power Input AC100-240V, 50/60Hz, 200W AC100-240V, 50/60Hz, 200W AC100-240V, 50/60Hz, 200W
Weight Net Weight 52 Kg (Approximately) 52 Kg (Approximately) 52 Kg (Approximately)

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